کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
641578 1457004 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous aqueous two-phase extraction and saponification reaction of chlorophyll from silkworm excrement
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Simultaneous aqueous two-phase extraction and saponification reaction of chlorophyll from silkworm excrement
چکیده انگلیسی


• The simultaneous ATPE and saponification reaction of chlorophyll was investigated.
• Conventional method was compared with simultaneous ATPE and saponification.
• Effects of parameters on the amount of sodium chlorophyllin were studied.
• The top system of the ATPS was used to prepare sodium copper chlorophyllin.

Simultaneous aqueous two-phase extraction and saponification reaction of chlorophyll from silkworm excrement was investigated in this study, and it was compared with the saponification reaction followed by aqueous two-phase extraction (ATPE) method. The results indicate that it is better to use the simultaneous aqueous two-phase extraction and saponification reaction method than other method. The effects of different parameters on the amount of sodium chlorophyllin in the top system of the ethanol–NaOH aqueous two-phase system (ATPS) were studied, including salt concentration, saponification temperature and time. When the NaOH concentration was 0.4 g/ml, saponification temperature and time were 333.15 K and 2 h respectively, the amount of sodium chlorophyllin in the top system reached its maximum in the investigated range. Further, the top system of the ethanol–NaOH ATPS was taken out and used to prepare sodium copper chlorophyllin, and the absorbance ratio (A405.00nm/A627.00nm) was 3.67.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 115, 30 August 2013, Pages 51–56
نویسندگان
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