Interaction of catalase with carrageenan applied to its recovery from murine liver

The complexes formation between an acid polyelectrolyte: carrageenan with catalase was evaluated in order to understand the mechanism of their interaction. Catalase was selected as the model enzyme since it is applied in several biotechnological processes. The study of this interaction had showed that the non-soluble complexes take place in a pH interval which depends on the salt presence. Carrageenan showed to be an appropriate option for the catalase precipitation, since the amount needed for the full precipitation of the enzyme was low, with a fast kinetic and a slight stabilization of the protein structure. After establishing the optimal conditions, we were able to purify catalase from murine liver, a natural source, with a purification factor of 6.4 and a yield of 19%, using only one step in the enzyme purification process.