کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
641706 1457009 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aqueous two-phase system for citrinin extraction from fermentation broth
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Aqueous two-phase system for citrinin extraction from fermentation broth
چکیده انگلیسی


• Aqueous two-phase system (ATPS) is original and pioneer for citrinin extraction.
• A green extraction process, ATPS (PEG/phosphate salts), for mycotoxin.
• PRDC-clean technology continuous extraction process for citrinin analysis.
• ATPS and PRDC are efficient extraction alternatives for citrinin, yield of 98.4%.

Aqueous two-phase systems (ATPSs) were investigated for effective extraction of citrinin from Penicillium citrinum fermentation broth. The citrinin partitioning behaviour in each ATPS (polyethylene glycol–phosphate salts) was evaluated in terms of the following parameters: polyethylene glycol (PEG) molecular weight, pH and tie line length. The target molecule (citrinin) presented high affinity to the PEG rich phase in most of the experiments. The best recovery yield (98.4%) was obtained using PEG 4000 (20% w/w)/phosphate salt (16.7% w/w), pH 3.4 at room temperature (28–30 °C), but the best citrinin partitioning (36.41) was observed at pH 5.7 using the same ATPS. Continuous citrinin extraction studies were performed using the fermentation broth and this ATPS in a perforated rotating disc contactor (PRDC). Recovery up to 98.44% supports the possible use of this system on a laboratory scale.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 110, 7 June 2013, Pages 158–163
نویسندگان
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