کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
642826 884341 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Partition behavior and partition mechanism of antibiotics in ethanol/2-propanol–ammonium sulfate aqueous two-phase systems
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Partition behavior and partition mechanism of antibiotics in ethanol/2-propanol–ammonium sulfate aqueous two-phase systems
چکیده انگلیسی

In this paper, the feasibility of applying the water-miscible alcohol–salt aqueous two-phase system to separate small molecular antibiotics in aqueous solution was proved. The partition behavior of tetracycline hydrochloride in the ethanol/2-propanol–(NH4)2SO4 aqueous two-phase system was investigated by determining partition coefficients and extraction efficiency. The partition coefficient and extraction efficiency of tetracycline hydrochloride in the ethanol–(NH4)2SO4 ATPS could reach 12.51% and 75.77% respectively at pH 2.00 and 0.50 g/mL (NH4)2SO4, furthermore, that of tetracycline hydrochloride in the 2-propanol–(NH4)2SO4 ATPS could reach 30.03% and 87.70% respectively at pH 1.52 and 0.50 g/mL (NH4)2SO4. The target was found to partition preferentially into the alcohol-rich phase in its cation form at pH < 4.0. The effects of the concentration of salt, the type of alcohol, pH and temperature were discussed in detail. The partition of target to the top phase was enhanced by decreasing pH from its isoelectric point of 5.4 and increasing the concentration of salt and temperature. Another objective of this study was to discuss the underlying mechanism governing the uneven partition of target and phase-separation. The experimental phenomenon and results both indicate that the salting-out effect of salt is the driving force for the uneven partition of target, so the salting-out effect of salts on the separation of target cannot be neglected in discussing aqueous two-phase extraction mechanism.

Figure optionsDownload as PowerPoint slideResearch highlights▶ The feasibility of applying the water-miscible alcohol–salt ATPS to separate small molecular antibiotics was proved. ▶ TC·HCl was found to partition preferentially into the top phase in its cation form. ▶ The salting-out effect of salts is the driving force for the formation of ATPS and the uneven partition of target.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 75, Issue 3, 20 November 2010, Pages 352–357
نویسندگان
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