کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
643450 | 884373 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Host–guest extraction of immunoglobulin G using calix[6]arenas Host–guest extraction of immunoglobulin G using calix[6]arenas](/preview/png/643450.png)
Host–guest interactions have been widely employed in various techniques in biochemistry in the last decades. In this way, the use of calix[6]arenes modified with a carboxylic acid as host in the host–guest extraction of immunoglobulin G (IgG) is studied. It is possible to extract IgG from an aqueous medium. Different operational parameters such as pH, salt concentration and stirring speed were evaluated. At high pH, up to 50% of the IgG can be transferred to the organic phase. The study of pH demonstrates that the interaction mechanism depends on a proton exchange mechanism together with hydrogen-bonding. Salt concentration has a direct effect on the interaction strength of the lysine residues of IgG and the calix[6]arene, leading to a diminished extraction at high salt concentrations. For the prevention of denaturation the contact mode and intensity of mixing are crucial. Based on the data obtained from kinetic and equilibrium studies a suggestion for the extraction mechanism is made.
Journal: Separation and Purification Technology - Volume 65, Issue 1, 2 February 2009, Pages 73–78