کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
644402 884445 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of spacer-arm and Cu(II) ions on performance of l-histidine immobilized on poly(GMA/MMA) beads as an affinity ligand for separation and purification of IgG
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Effect of spacer-arm and Cu(II) ions on performance of l-histidine immobilized on poly(GMA/MMA) beads as an affinity ligand for separation and purification of IgG
چکیده انگلیسی

In this study, the beads were prepared from glycidiyl methacrylate (GMA) and methyl methacrylate (MMA) via suspension polymerization and, the used beads fractions were between 75 and 150 μm. The epoxy groups of the beads were converted into amino groups by the reaction of ammonia or 1,6-diaminohexane as a spacer-arm. l-Histidine ligand was immobilized onto both beads. Cu(II) ions were chelated onto spacer-arm attached and l-histidine immobilized beads. The IgG adsorption capacity of the spacer-arm attached and Cu(II) chelated affinity beads led to higher adsorption capacities about 1.64- and 2.94-fold, respectively. The adsorption equilibrium studies showed that the adsorption isotherm of IgG obeyed the Langmuir isotherm model. The experimental data was well described by the second-order equations. Purification data of IgG with spacer-arm attached and Cu(II) ions chelated (i.e. poly(GMA/MMA)-SAH-Cu(II)) beads indicated that 87.5% of IgG was removed from human serum with a purity of 90%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 50, Issue 2, 15 June 2006, Pages 229–239
نویسندگان
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