کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6450562 1416127 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of adipose stem cell sheets on colon anastomotic leakage in an experimental model: Proof of principle
ترجمه فارسی عنوان
اثرات ورق های سلول های بنیادی بافت چربی بر نشت آناستوموتیک کولون در یک مدل تجربی: اثبات اصل
کلمات کلیدی
سلول های بنیادی حاصل از بافت چربی، ورق سلولی، نشت انستوموتیک، شفا،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

The most dreaded complication of colorectal surgery is anastomotic leakage. Adipose tissue-derived stem cell sheets (ASC sheets) prepared from temperature-responsive culture surfaces can be easily transplanted onto tissues. These sheets are proposed to improve cell transplant efficiency and enhance wound healing. The aim of this study was to investigate whether application of ASC sheets could prevent leakage of sutured colorectal anastomoses. Insufficient suturing of colorectal anastomoses was performed in Wistar rats to create a colorectal anastomotic leakage model. Rats were randomized to ASC sheet application or control group. Leakage, abscess formation, adhesion formation, anastomotic bursting pressure (ABP), and histology were evaluated on postoperative day 3 or 7. ASC sheet application significantly reduced anastomotic leakage compared to controls, without increased adhesion formation. ASC sheet transplantation resulted in more CD3+ T-cells and CD163+ anti-inflammatory macrophages at the anastomotic site than the control group. ABP, vessel density and collagen deposition were not different between groups. Using cell sheet technology, we generated ASC sheets that prevented disruption of sutured colorectal anastomoses as shown by reduced leakage. Increased numbers of anti-inflammatory macrophages and T-cells might have contributed to this positive effect.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 140, September 2017, Pages 69-78
نویسندگان
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