کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6450931 1416152 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
YAP-dependent mechanotransduction is required for proliferation and migration on native-like substrate topography
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
YAP-dependent mechanotransduction is required for proliferation and migration on native-like substrate topography
چکیده انگلیسی

Native vascular extracellular matrices (vECM) consist of elastic fibers that impart varied topographical properties, yet most in vitro models designed to study the effects of topography on cell behavior are not representative of native architecture. Here, we engineer an electrospun elastin-like protein (ELP) system with independently tunable, vECM-mimetic topography and demonstrate that increasing topographical variation causes loss of endothelial cell-cell junction organization. This loss of VE-cadherin signaling and increased cytoskeletal contractility on more topographically varied ELP substrates in turn promote YAP activation and nuclear translocation, resulting in significantly increased endothelial cell migration and proliferation. Our findings identify YAP as a required signaling factor through which fibrous substrate topography influences cell behavior and highlights topography as a key design parameter for engineered biomaterials.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 115, January 2017, Pages 155-166
نویسندگان
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