Short communicationStructured interlocked-microcapsules: A novel scaffold for enzyme immobilization

- Novel structured interlocked-microcapsules (SIMC) was synthesized.
- The enzyme-immobilized SIMC presented high catalytic performance.
- The enzyme-immobilized SIMC exhibited significantly enhanced stability.
- Internal diffusion resistance was reduced due to the unique structure.

A facile approach to prepare structured interlocked-microcapsules (SIMC) was developed, which combined the advantages of open mouthed structure, hierarchical porous nanostructure and interlocked architecture. The specific surface area of SIMC was 374.6 m2/g and the diameter of the pores was 8.707 nm. Nitrile hydratase (NHase) was immobilized on SIMC via covalent bonding to realize the easy separation of the enzyme and improve properties of enzyme such as pH tolerance and heat stability. This work demonstrated that the enzyme-immobilized SIMC presented high catalytic performance and significantly improved stability.

305