کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6462205 1421972 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Zopiclone concentrations in oral fluid and blood after, administration of therapeutic doses of zopiclone
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Zopiclone concentrations in oral fluid and blood after, administration of therapeutic doses of zopiclone
چکیده انگلیسی


- Oral fluid zopiclone concentration is unsuitable to estimate blood concentration.
- Intake of a meal reduces the oral fluid to blood concentration ratio.
- Intake of 10 mg zopiclone can be detected in oral fluid up to 14 days.

PurposeLittle is known about the relationship between concentrations in oral fluid (OF) and blood for the widely prescribed hypnotic drug zopiclone. The purpose of this study was to investigate the usefulness of OF zopiclone concentrations to predict blood zopiclone concentrations in order to introduce OF testing as an alternative to more cumbersome blood testing.Methods16 healthy young male volunteers received capsules of either 5 or 10 mg zopiclone on two different study days separated by at least one week. Blood and OF were collected simultaneously at baseline and 9 times after intake of zopiclone on each study day. In addition an OF sample was collected 24-81 h after intake. Lunch was served between samples taken 2.5 and 3.5 h after intake. All samples were analysed for zopiclone, and the cut-off was 10 ng/ml in blood and 0.2 ng/ml in OF-buffer mixture.ResultsZopiclone was detected in all OF samples during the study day. After 24-81 h, all subjects were also positive for zopiclone in OF, except from three subjects ingesting the 5 mg dose. In a single case zopiclone was detected in a baseline OF sample 14 days after intake on an earlier study day. Zopiclone was detected in both OF and blood in 231 OF/blood pairs, and a significant but weak correlation between OF and blood concentration was seen (R2 of 0.30). The median (range) zopiclone OF/blood concentration ratio (ZOBCR) for all samples were 3.3 (0.8-18). The ZOBCR decreased when the OF volume increased. After 30 of 31 given doses of zopiclone, the ZOBCR was higher in samples collected before lunch than samples collected after lunch.DiscussionVast intra- and interindividual differences in ZOBCR were found, and the correlation between OF and blood concentration was less pronounced than reported in former studies. In accordance with earlier studies we found a negative correlation between ZOBCR and OF volume. The ZOBCR decreases in relation to recent intake of a meal, probably because stimulated saliva production causes “dilution” of saliva. OF zopiclone concentration appeared unsuitable for estimation of blood zopiclone concentration. Due to long detection time, analysis of zopiclone in OF might be useful to detect non-recent, previous intake.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International - Volume 278, September 2017, Pages 177-183
نویسندگان
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