کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6481819 1401402 2016 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Guidelines for the selection of functional assays to evaluate the hallmarks of cancer
ترجمه فارسی عنوان
دستورالعمل برای انتخاب آزمونهای عملکردی برای ارزیابی نشانه های سرطان
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی


- Selecting appropriate in vitro assays to monitor hallmarks of cancer is challenging.
- We cover frequently used methods for analyzing each hallmark.
- Principles, advantages, and limitations are discussed for each method.
- Procedures supporting the study of multiple hallmarks simultaneously are highlighted.

The hallmarks of cancer capture the most essential phenotypic characteristics of malignant transformation and progression. Although numerous factors involved in this multi-step process are still unknown to date, an ever-increasing number of mutated/altered candidate genes are being identified within large-scale cancer genomic projects. Therefore, investigators need to be aware of available and appropriate techniques capable of determining characteristic features of each hallmark.We review the methods tailored to experimental cancer researchers to evaluate cell proliferation, programmed cell death, replicative immortality, induction of angiogenesis, invasion and metastasis, genome instability, and reprogramming of energy metabolism. Selecting the ideal method is based on the investigator's goals, available equipment and also on financial constraints. Multiplexing strategies enable a more in-depth data collection from a single experiment - obtaining several results from a single procedure reduces variability and saves time and relative cost, leading to more robust conclusions compared to a single end point measurement. Each hallmark possesses characteristics that can be analyzed by immunoblot, RT-PCR, immunocytochemistry, immunoprecipitation, RNA microarray or RNA-seq. In general, flow cytometry, fluorescence microscopy, and multiwell readers are extremely versatile tools and, with proper sample preparation, allow the detection of a vast number of hallmark features. Finally, we also provide a list of hallmark-specific genes to be measured in transcriptome-level studies.Although our list is not exhaustive, we provide a snapshot of the most widely used methods, with an emphasis on methods enabling the simultaneous evaluation of multiple hallmark features.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Reviews on Cancer - Volume 1866, Issue 2, December 2016, Pages 300-319
نویسندگان
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