کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6484431 | 1416090 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Extracellular fluid viscosity enhances liver cancer cell mechanosensing and migration
ترجمه فارسی عنوان
ویسکوزیته مایع خارج سلولی باعث تقویت مکانیزم و مهاجرت سلول های سرطانی کبدی می شود
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کلمات کلیدی
سرطان، میکرو محیط، ویسکوزیته، مکانیک انتقال، مهاجرت،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The extracellular fluid (ECF) is a crowded environment containing macromolecules that determine its characteristic density, osmotic pressure, and viscosity, which greatly differ between tissues. Precursors and products of degradation of biomaterials enhance ECF crowding and often increase its viscosity. Also, increases in ECF viscosity are related to mucin-producing adenocarcinomas. However, the effect of ECF viscosity on cells remains largely unexplored. Here we show that viscosity-enhancing polymer solutions promote mesenchymal-like cell migration in liver cancer cell lines. Also, we demonstrate that viscosity enhances integrin-dependent cell spreading rate and causes actin cytoskeleton re-arrangements leading to larger cell area, nuclear flattening, and nuclear translocation of YAP and β-catenin, proteins involved in mechanotransduction. Finally, we describe a relationship between ECF viscosity and substrate stiffness in determining cell area, traction force generation and mechanotransduction, effects that are actin-dependent only on ⤠40â¯kPa substrates. These findings reveal that enhancing ECF viscosity can induce major biological responses including cell migration and substrate mechanosensing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 177, September 2018, Pages 113-124
Journal: Biomaterials - Volume 177, September 2018, Pages 113-124
نویسندگان
Jordi Gonzalez-Molina, Xiaoli Zhang, Michela Borghesan, Joana Mendonça da Silva, Maooz Awan, Barry Fuller, Núria Gavara, Clare Selden,