کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6484452 1416092 2018 43 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Carbon nanotube multilayered nanocomposites as multifunctional substrates for actuating neuronal differentiation and functions of neural stem cells
ترجمه فارسی عنوان
نانوکامپوزیتهای چند لایه نانولوله کربنی به عنوان زیربنای چند منظوره برای فعال کردن تمایز عصبی و عملکرد سلول های بنیادی عصبی
کلمات کلیدی
چند لایه نانولوله کربنی، سلول های بنیادی عصبی، تفکیک، توابع، مکانیزم های مولکولی،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Carbon nanotubes (CNTs) have shown potential applications in neuroscience as growth substrates owing to their numerous unique properties. However, a key concern in the fabrication of homogeneous composites is the serious aggregation of CNTs during incorporation into the biomaterial matrix. Moreover, the regulation mechanism of CNT-based substrates on neural differentiation remains unclear. Here, a novel strategy was introduced for the construction of CNT nanocomposites via layer-by-layer assembly of negatively charged multi-walled CNTs and positively charged poly(dimethyldiallylammonium chloride). Results demonstrated that the CNT-multilayered nanocomposites provided a potent regulatory signal over neural stem cells (NSCs), including cell adhesion, viability, differentiation, neurite outgrowth, and electrophysiological maturation of NSC-derived neurons. Importantly, the dynamic molecular mechanisms in the NSC differentiation involved the integrin-mediated interactions between NSCs and CNT multilayers, thereby activating focal adhesion kinase, subsequently triggering downstream signaling events to regulate neuronal differentiation and synapse formation. This study provided insights for future applications of CNT-multilayered nanomaterials in neural fields as potent modulators of stem cell behavior.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 175, August 2018, Pages 93-109
نویسندگان
, , , , , , , , , , ,