کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6485256 | 395 | 2016 | 51 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Orthotopic transplantation of a tissue engineered diaphragm in rats
ترجمه فارسی عنوان
پیوند ارتوتوپی دیافراگم مهندسی بافت در موش صحرایی
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کلمات کلیدی
مهندسی بافت، داربست، دیافراگم، پیوند زیست سازگاری،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The currently available surgical options to repair the diaphragm are associated with significant risks of defect recurrence, lack of growth potential and restored functionality. A tissue engineered diaphragm has the potential to improve surgical outcomes for patients with congenital or acquired disorders. Here we show that decellularized diaphragmatic tissue reseeded with bone marrow mesenchymal stromal cells (BM-MSCs) facilitates in situ regeneration of functional tissue. A novel bioreactor, using simultaneous perfusion and agitation, was used to rapidly decellularize rat diaphragms. The scaffolds retained architecture and mechanical properties and supported cell adhesion, proliferation and differentiation. Biocompatibility was further confirmed in vitro and in vivo. We replaced 80% of the left hemidiaphragm with reseeded diaphragmatic scaffolds. After three weeks, transplanted animals gained 32% weight, showed myography, spirometry parameters, and histological evaluations similar to native rats. In conclusion, our study suggested that reseeded decellularized diaphragmatic tissue appears to be a promising option for patients in need of diaphragmatic reconstruction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 77, January 2016, Pages 320-335
Journal: Biomaterials - Volume 77, January 2016, Pages 320-335
نویسندگان
Elena A. Gubareva, Sebastian Sjöqvist, Irina V. Gilevich, Alexander S. Sotnichenko, Elena V. Kuevda, Mei Ling Lim, Neus Feliu, Greg Lemon, Konstantin A. Danilenko, Ramazan Z. Nakokhov, Ivan S. Gumenyuk, Timofei E. Grigoriev, Sergey V. Krasheninnikov,