کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6485918 415 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo
چکیده انگلیسی
Endogenous hypochlorite ion (ClO−) is a highly reactive oxygen species (ROS) that is produced from hydrogen peroxide and chloride ions catalyzed by myeloperoxidase (MPO). And mitochondrion is one of the major sources of ROS including ClO−. In the present work, a two-photon phosphorescent probe for ClO− in mitochondria was developed. An iridium(III) complex bearing a diaminomaleonitrile group as ClO− reactive moiety specifically responded to ClO− over other ions and ROSs. When the probe was reacted with ClO− to form an oxidized carboxylate product, a significant enhancement in phosphorescence intensity was observed under one-photon (402 nm) and two-photon (750 nm) excitation, with a two-photon absorption cross-section of 78.1 GM at 750 nm. More importantly, ICP-MS results and cellular images co-stained with Mito-tracker Green demonstrated that this probe possessed high specificity for mitochondria. This probe was applied in the one- and two-photon imaging of ClO− in vitro and in vivo. The results suggested endotoxin lipopolysaccharide (LPS) induced ClO− mostly generated in the liver of zebrafish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 53, June 2015, Pages 285-295
نویسندگان
, , , , , , , , , , ,