کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6486118 418 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effects of electrospun substrate-mediated cell colony morphology on the self-renewal of human induced pluripotent stem cells
ترجمه فارسی عنوان
اثرات مورفولوژی کلونی سلول های بکار رفته توسط الکترواسپون بر روی خود تجدید سلول های بنیادی پلورپوفتون القا شده توسط انسان
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The development of xeno-free, chemically defined stem cell culture systems has been a primary focus in the field of regenerative medicine to enhance the clinical application of pluripotent stem cells (PSCs). In this regard, various electrospun substrates with diverse physiochemical properties were synthesized utilizing various polymer precursors and surface treatments. Human induced pluripotent stem cells (IPSCs) cultured on these substrates were characterized by their gene and protein expression to determine the effects of the substrate physiochemical properties on the cells' self-renewal, i.e., proliferation and the maintenance of pluripotency. The results showed that surface chemistry significantly affected cell colony formation via governing the colony edge propagation. More importantly, when surface chemistry of the substrates was uniformly controlled by collagen conjugation, the stiffness of substrate was inversely related to the sphericity, a degree of three dimensionality in colony morphology. The differences in sphericity subsequently affected spontaneous differentiation of IPSCs during a long-term culture, implicating that the colony morphology is a deciding factor in the lineage commitment of PSCs. Overall, we show that the capability of controlling IPSC colony morphology by electrospun substrates provides a means to modulate IPSC self-renewal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 50, May 2015, Pages 10-19
نویسندگان
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