کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6551784 160413 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive liquid chromatography/tandem mass spectrometry method for the simultaneous determination of nine local anesthetic drugs
ترجمه فارسی عنوان
روش کروماتوگرافی مایع حساس / روش اسپکترومتری توده دو طرفه برای تعیین همزمان 9 داروهای بیهوشی محلی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) with electrospray ionization (ESI) procedure for the simultaneous determination of nine local anesthetic drugs (procaine, mepivacaine, lidocaine, ropivacaine, oxybuprocaine, tetracaine, bupivacaine, T-caine and dibucaine) in human serum is described. The chromatographic separation was performed on a Mightysil-RP-18 GP II column (2.0 mm × 150 mm, particle size 5 μm). The mobile phase consisted of 10 mM acetic ammonium buffer (pH 5.4) and acetonitrile and was delivered at a flow rate of 0.20 mL/min. The triple quadrupole mass spectrometer was operated in positive ion mode, and multiple reaction monitoring was used for drug quantification. Solid-phase extraction of the nine local anesthetic drugs added to the human serum was performed with an Oasis® HLB extraction cartridges column. The method was linear for the investigated drugs over the concentration range of 10-100 ng/mL. The recoveries of these drugs were in the range of 81.4-144%. The standard deviation (SD) values for all analytes were <0.10 for both intraday and interday accuracy and precision. The selectivity, accuracy and precision of this method are satisfactory for clinical and forensic applications. The sensitive and selective method offers the opportunity for the simultaneous screening and quantification, for clinical and forensic purposes, of almost all local anesthetics available in Japan.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International - Volume 265, August 2016, Pages 182-185
نویسندگان
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