کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6555921 | 1422499 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel messenger RNAs for body fluid identification
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کلمات کلیدی
Capillary electrophoresis - الکتروفورزمویرگیGene expression patterns - الگوهای بیان ژنTranscriptome sequencing - توالی ترانسپرتیموتیmessenger ribonucleic acid - رسوب ریبونوکلئیک اسیدBody fluid identification - شناسایی مایع بدنForensic science - علم پزشکی قانونیSeminal fluid - مایع معدهpolymerase chain reaction - واکنش زنجیره ای پلیمرازsemen - گُشناب یا منی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Here we characterise six novel mRNAs, partly identified from RNA-Seq, which can supplement existing markers for the detection of circulatory blood, semen (with and without spermatozoa), and menstrual fluid: HBD and SLC4A1 for blood, TNP1 for spermatozoa, KLK2 for seminal fluid, and MMP3 and STC1 for menstrual fluid. Respective expression profiles were evaluated by singleplex endpoint reverse transcription polymerase chain reaction (RT-PCR). HBD, SLC4A1, and KLK2 were specific to their target body fluids. TNP1, MMP3, and STC1 each cross-reacted with two non-target samples; however, these signals were below 350Â RFU, not reproducible, and likely resulted from large body fluid inputs. All candidates were more sensitive for the detection of their target body fluids than corresponding well-known mRNAs, in particular those for menstrual fluid. The increased sensitivities were statistically significant, except for KLK2. Thus, the new mRNAs introduced here are promising new targets for improved body fluid profiling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science & Justice - Volume 58, Issue 2, March 2018, Pages 145-152
Journal: Science & Justice - Volume 58, Issue 2, March 2018, Pages 145-152
نویسندگان
Patricia P. Albani, Rachel Fleming,