Genome-scale in silico modeling and analysis for designing synthetic terpenoid-producing microbial cell factories

► Developed in silico rational framework for synthetic terpenoid production. ► Updated metabolic model of A. thaliana to describe terpenoid biosynthesis. ► Analyzed heterologous expression of A. thaliana pathways in microbial systems. ► Proposed novel in silico method of cofactor modification analysis. ► Improve terpenoid yield by modifying enzyme cofactor specificity.