Optimization of digested chicken manure filtrate supplementation for lipid overproduction in heterotrophic culture Chlorella protothecoides

► Digested chicken manure filtrate (DCMF) was used to promote lipid production in C. protothecoides. ► Feeding higher nitrogen DCMF at cell growth phase enhanced cell concentration. ► Feeding lower nitrogen DCMF at stationary phase improved lipid content.. ► The strategies of DCMF supplementation coupled with glucose feeding maximized lipid production.