Concomitant extraction of bio-oil and value added polysaccharides from Chlorella sorokiniana using a unique sequential hydrothermal extraction technology

► Method uses temperature gradient for sequential extraction of desired products. ► This method successively produce polysaccharides and bio-oil from algal biomass. ► Method used, produces 63% less bio char than conventional method. ► First report of bio-oil production from heterotrophically grown Chlorella sorokiniana.