Simultaneous gas chromatographic analysis of total esters, mono-, di- and triacylglycerides and free and total glycerol in methyl or ethyl biodiesel

► The simultaneous GC analysis of total esters, acylglycerides and glycerol was developed. ► The method provides separation of acylglycerides, methyl and ethyl esters peaks. ► It was used C23:0 as internal standard. ► The method developed is faster and allows the elution of long-chain triacylglycerides.