Bioethanol production from autohydrolyzed Eucalyptus globulus by Simultaneous Saccharification and Fermentation operating at high solids loading

► This work deals with the utilization of Eucalyptus globulus as substrate for 2nd generation biofuels production. ► Eucalyptus globulus was pretreated in aqueous media and then subjected to Simultaneous Saccharification and Fermentation. ► The operational conditions were optimized on the basis of a Response Surface Methodology assessment. ► The whole processing scheme resulted in high ethanol yields, even in operation at high substrate loading.