Extraction of polar lipids from bifidobacteria by supercritical carbon dioxide (scCO2)

► Supercritical fluid extraction of Bifidobacterium longum and Bifidobacterium angulatum, strains with probiotic properties. ► The polar lipids were isolated using the following conditions: pressure 250 bar, temperature 45 °C, CO2 flow rate 5 g/min, hydromethanolic co-solvent concentration 10% (v/v). ► Yields and compositions of polar lipids of different extracts were evaluated. ► On comparison with classical method, extraction with scCO2 yielded more polar lipids.