Stability, activity, and selectivity of benzaldehyde lyase in supercritical fluids

► Benzaldehyde lyase (BAL) activity, enantioselectivity, and stability depends on enzyme preparation, supercritical fluid, and water activity (aw). ► Increase of aw decreases residual stability but increases enantioselectivity. ► The preparation pH of the lyophilisate is most important for residual stability and is highest at pH = 6.5.