کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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676337 | 1459739 | 2014 | 5 صفحه PDF | دانلود رایگان |
Apoptosis in oocyte could be a good marker for oocyte quality and development competency. The study aims to investigate the relation between early apoptosis occurrence in different morphological groups of oocytes, i.e. Group A, B and C, and their developmental potential in terms of meiotic resumption to metaphase II. Annexin-V staining was used to detect early apoptosis in oocytes and Giemsa staining for meiotic resumption. Immature oocytes in Group B and C showed significantly high incidence of early apoptosis compared to Group A oocytes (A: 10.20%, B: 19.00% and C: 20.60%). After maturation, no differences were observed in the incidence of early apoptosis among oocytes from different groups (A: 28.40%, B: 18.20% and C: 23.00%). However, the mean percentage of early apoptosis increased among Group A oocytes after maturation. The progression to metaphase II were similar among the different groups of oocytes (A: 34.09%, B: 31.54% and C: 33.45%). In conclusion, early apoptosis occurrence in bovine oocytes is related to developmental competence.
Journal: APCBEE Procedia - Volume 8, 2014, Pages 272-276