Microbial oil production by Mortierella isabellina from sodium hydroxide pretreated rice straw degraded by three-stage enzymatic hydrolysis in the context of on-site cellulase production

Lignocellulosic single cell oil (SCO) is profound and promising in response to the increasing concerns on sustainable energy supply and environmental protection. Different bioprocesses from sodium hydroxide pretreated rice straw (SHPRS) to SCO were compared and the bioprocess c using the three-stage enzymatic hydrolysis was found to be the most efficient. It had the lowest enzyme input 153.1 FPIU cellulase/g SCO and the shortest time 222 h, but produced 42.0 g dry cell biomass and 23.4 g SCO from 342.0 g dry SHPRS. It had the highest lipid content 55.7%, and its productivities and yields were the highest. This study verified that on-site cellulase production by the mixed culture of Trichoderma reesei and Aspergillus niger and the three-stage enzymatic hydrolysis have the application value in SCO production from lignocelluloses by Mortierella isabellina.