کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6765308 1431590 2018 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hydrogen production by Escherichia coli using brewery waste: Optimal pretreatment of waste and role of different hydrogenases
موضوعات مرتبط
مهندسی و علوم پایه مهندسی انرژی انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
Hydrogen production by Escherichia coli using brewery waste: Optimal pretreatment of waste and role of different hydrogenases
چکیده انگلیسی
Brewery spent grains (BSG), one of the by-products of brewery production, were used for Escherichia coli growth and hydrogen (H2) production. The dilute acid and alkali pretreatment methods were used to hydrolyze the rough lignocellulose structure, and optimal conditions for the BSG hydrolysate (BSGH) preparation were developed. E. coli BW25113 wild type strain and hydrogenase (Hyd)-negative mutants with deletions of genes encoding key subunits of Hyd 1-4 (ΔhyaB, ΔhybC, ΔhycE, ΔhyfG), as well as for a ΔhyaB ΔhybC double mutant were investigated with regards to growth, acidification of the medium, redox potential kinetics and H2 production when using BSGH. Readings of redox Pt electrode dropped to −400 ± 10 mV, with H2 yield of ∼0.75 mmol H2 L−1 at the 3rd h wild type strain growth. Changes in redox Ti-Si electrode readings were negligible. H2 production was not observed with defective Hyd-3 and Hyd-4; therefore, Hyd-3 and Hyd-4 are responsible for H2 production using BSGH, whereas defective Hyd-1 and Hyd-2 led to a ∼2-fold stimulation of H2 yield. The data were confirmed by determining cumulative H2 yield. These findings are useful for development of renewable energy, especially H2 production biotechnology, using different organic wastes as sustainable energy feedstocks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Renewable Energy - Volume 115, January 2018, Pages 931-936
نویسندگان
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