Enhancement of D-amino Acid Oxidase By Trigonopsis Variabilis

Goal of present studies was the optimization of production media of D-amino acid oxidase by Trigonopsis variabilis using one-at-a-time strategy & screening of novel inducer. In different carbon and nitrogen sources, glucose and ammonium sulfate were found the best carbon and nitrogen source with 4:1 C: N ratio. The best new inducers found were 3-(3, 4 -Dihydroxyphenyl) DL-alanine, DL-β- Phenyl alanine, DL-2- Amino-n-butyric acid. Four fold of D-amino acid oxidase yield was observed when 3-(3, 4 -Dihydroxyphenyl) DL-alanine was used as inducer as compared with D-Ala. The optimum concentration of the best inducer 3-(3, 4 -Dihydroxyphenyl) DL-alanine, was 10 mM. High Induction effect was observed when inducer was added in media in 0 hour of fermentation. © 2012 Published by Elsevier B.V. Selection and/or peer review under responsibility of Asia-Pacific Chemical, Biological & Environmental Engineering Society