Optimization of Process Parameters for ɛ-PolyLysine Production from Mixed Carbon Source using “One Factor at a Time” Technique

The goal of this study was to improve the efficiency of ɛ-polylysine production by Streptomyces albulus using glycerol and glucose as carbon source. The various physico-chemical parameters were studied for ɛ-PL production and optimized using “one factor at a time” technique. The initial weight ratio of glucose to glycerol played an important role and a weight ratio of 1:1 resulted in maximum production of ɛ-PL production by S. albulus. Under optimum condition 1.2 g/l of ɛ-PL was obtained in mixed carbon source by wild strain of S. albulus.