Compositional changes in industrial hemp biomass (Cannabis sativa L.) induced by electron beam irradiation Pretreatment

The effects of electron beam irradiation on chemical decomposition of industrial hemp biomass were evaluated at doses of 150, 300, and 450 kGy. The quantity of decomposed components was indirectly estimated by measuring changes in alkaline extraction. The more severe degradation of structural components induced by higher irradiation doses resulted in larger amounts of alkaline extract. Carbohydrate compositional analysis using 1H-NMR spectroscopy was applied to quantitatively investigate changes in the polysaccharides of the industrial hemp. The xylose peak intensity in the NMR spectra decreased with increasing electron irradiation dose, indicating that xylan was more sensitive to electron beam irradiation than cellulose.

► The more severe degradation of structural components induced by higher irradiation.
► Carbohydrate analysis was applied to quantitatively investigate changes in the industrial hemp.
► Xylan was more sensitive to electron beam irradiation than cellulose.