Interactions of acetylcholine binding site residues contributing to nicotinic acetylcholine receptor gating: Role of residues Y93, Y190, K145 and D200

- Interactions among αY93, αY190, αK145 and αD200 trigger opening and closing of loop C.
- The αγ and the αδ ACh binding sites exhibited distinct behavior.
- Fluorescent binding assays show agonist preferentially binds to the αγ binding site.