Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology

► Screening of mixotrophic nutrients for biomass production by C. vulgaris using RSM. ► Glucose, MgSO4, KNO3 and NaCl are the critical mixotrophic nutrients of C. vulgaris. ► Mixotrophic C. vulgaris biomass can be used as the raw material for biorefinery.