کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
691526 1460430 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous degradation of organophosphorus and organochlorine pesticides by bacterial consortium
ترجمه فارسی عنوان
تخریب همزمان آفت کش های ارگانوفسفره و آلی کلرور توسط کنسرسیوم باکتریایی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی


• Biodegradation of organophosphorus and organochlorine pesticides are being reported.
• A laboratory scale fermentor–bioreactor system was used in the degradation process.
• Bacterial consortium was able to completely degrade the pesticides within 24 h of incubation.
• The toxic metabolites TCP and endosulfan sulfate were undetected indicating successful degradation.

Several researchers have focussed on degradation of pesticides by microorganisms, which is the most viable option in remediation of agricultural soil. However, very few have illustrated the degradation of mixed pesticides. Hence, in the present investigation biodegradation of a mixture of both organophosphorus and organochlorine pesticide directed by microbial consortium was carried out in a fermentor–bioreactor system. The pesticides chosen were chlorpyrifos, monocrotophos (organophosphorus) and endosulfan (organochlorine). In order to accomplish an effective degradation, the microorganisms were isolated from agricultural fields which were previously exposed to the pesticides mentioned above. The bacteria were isolated by minimal meda with the individual pesticide as the only carbon source. The individual strains were identified by morphological, biochemical and 16S rRNA characterization. The bacterial consortium comprised of 10 organisms which were as follows: Alcaligenes sp. JAS1, Ochrobactrum sp. JAS2, Sphingobacterium sp. JAS3 which were screened and selected from chlorpyrifos contaminated soil, Enterobacter ludwigii JAS17, Pseudomonas moraviensis JAS18 and Serratia marcescens JAS16 which were isolated from monocrotophos spiked soil and Klebsiella pneumoniae JAS8, Enterobacter cloacae JAS7, Halophilic bacteria JAS4, Enterobacter asburiae strain JAS5 were obtained from endosulfan persistent soil sample. The fermentor–bioreactor system containing 2 L of sterile minimal medium supplemented with 300 mg l−1 of chlorpyrifos, 1000 mg l−1 of monocrotophos, 1000 mg l−1 of endosulfan as the only source of carbon and inoculated with 20 ml of bacterial consortium (approximately 3 × 106 cells ml−1). The HPLC and GC–MS studies were performed to record the degradation of mixed pesticides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 45, Issue 5, September 2014, Pages 2590–2596
نویسندگان
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