کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
691714 1460451 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High throughput study of separation of poly(3-hydroxybutyrate) from recombinant Escherichia coli XL1 blue
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
High throughput study of separation of poly(3-hydroxybutyrate) from recombinant Escherichia coli XL1 blue
چکیده انگلیسی

Polyhydroxyalkanoates (PHAs) are biodegradable plastics. Currently most PHAs are produced using microbial fermentation. The separation of PHAs is a key factor in the design of a successful production scheme. This study chose a recombinant Escherichia coli to produce poly(3-hydroxybutyrate) (PHB), an important one among PHAs; and to use different separation methods to recover PHB. The diversity of PHB obtained by different separation methods was characterized. Among different methods used, sodium dodecyl sulfate (SDS)–NaOH method was selected as the best in separation from the results obtained from experiments using a combinatorial design. In the combinatorial design, only 16 runs are needed to generate 150 sets of experimental data. By using a two-stage design, PHB with a purity of 99% was obtained when the first-stage digestion used SDS digestion method (50 °C, 125 rpm, biomass/digestion solution (100 g/L) = 1/10 (w/v) and 10 min) and the second-stage used NaOH method (50 °C, 125 rpm, biomass/digestion solution (1 M) = 1/10 (w/v) and 10 min).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 42, Issue 2, March 2011, Pages 240–246
نویسندگان
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