کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6949 | 525 | 2012 | 9 صفحه PDF | دانلود رایگان |
We demonstrate bifunctional combined Au-Fe2O3 nanoparticles (NPs) for selectively induction of apoptosis in cancer cells and real-time imaging. The as-prepared Au-Fe2O3 NPs combine the merits of both Au and γ-Fe2O3 NPs, maintaining excellent fluorescence quenching property and catalytic activity. Conjugated with αⅤβ3 integrin-targeting peptide (RGD) and fluorescein isothiocyanate (FITC)-labeled capsase-3 recognition sequence (DEVD) on the Au surface, the resulting RGD/FITC–DEVD–Au-Fe2O3 NPs bind preferentially to integrin αⅤβ3-rich human liver cancer cells (HepG2), sequentially initiate catalytic formation of hydroxyl radicals (OH) and enable the real-time monitoring ofOH-induced caspase-3-dependent apoptosis in these cancer cells. Furthermore, the catalytic activity of RGD/FITC–DEVD–Au-Fe2O3 NPs is much higher than that of individual γ-Fe2O3 NPs due to the polarization effect at the Au-Fe2O3 interface. Such bifunctional Au-Fe2O3 NPs exhibit simultaneous targeting, therapeutic and imaging functions and are therefore promising for future therapeutic applications in cancer.
Journal: Biomaterials - Volume 33, Issue 14, May 2012, Pages 3710–3718