کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6952273 | 1451763 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Azoreductase kinetics and gene expression in the synthetic dyes-degrading Pseudomonas
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی کامپیوتر
پردازش سیگنال
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Microorganisms have an effective capability to degrade synthetic dyestuff enzymatically. In this study, two 16S rRNA identified Pseudomonas aeruginosa bacterial isolates (ASU3 and ASU6) have been screened to enzymatically degrade both Disperse Blue 64 (DB64) and Acid Yellow 17 (AY17) which resembles 2 of the synthetic dyes that causes aquatic environmental pollution. The expression of the azoR1 gene has been detected in the strains, the degradation percentage of DB64 and AY17 by stain ASU6 was found to exceed the ASU3. This returned to the differences in the expression levels of azoR1 between the ASU6 and the ASU3 which was confirmed by RT-PCR. In addition, the specific activity (U/mg protein) of azoreductase in ASU6 was higher than ASU3 when MethylRed (MR), DB64 and AY17 were used as a substrate, separately. The azoreductase kinetics was studied to find the optimum condition for maintaining the stability of the enzyme. The degraded products separated by HPLC/MS were tested for their toxicity on two crop plants and they were nontoxic to the plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Egyptian Journal of Basic and Applied Sciences - Volume 4, Issue 4, December 2017, Pages 315-322
Journal: Egyptian Journal of Basic and Applied Sciences - Volume 4, Issue 4, December 2017, Pages 315-322
نویسندگان
Ameer Elfarash, Asmaa M.M. Mawad, Naeima M.M. Yousef, Ahmed A.M. Shoreit,