Interactions between Microcystis aeruginosa and coexisting amoxicillin contaminant at different phosphorus levels

Microcystis aeruginosa was cultured with 0.05-5 mg L−1 of phosphorus and exposed to 200-500 ng L−1 of amoxicillin for seven days. Amoxicillin presented no significant effect (p > 0.05) on the growth of M. aeruginosa at phosphorus levels of 0.05 and 0.2 mg L−1, but stimulated algal growth as a hormesis effect at phosphorus levels of 1 and 5 mg L−1. Phosphorus and amoxicillin affected the contents of chlorophyll-a, adenosine triphosphate (ATP) and malondialdehyde, the expression of psbA and rbcL, as well as the activities of adenosinetriphosphatase and glutathione S-transferase in similar manners, but regulated the production and release of microcystins and the activities of superoxide dismutase and peroxidase in different ways. Increased photosynthesis activity was related with the ATP consumption for the stress response to amoxicillin, and the stress response was enhanced as the phosphorus concentration increased. The biodegradation of amoxicillin by M. aeruginosa increased from 11.5% to 28.2% as the phosphorus concentration increased. Coexisting amoxicillin aggravated M. aeruginosa pollution by increasing cell density and concentration of microcystins, while M. aeruginosa alleviated amoxicillin pollution via biodegradation. The interactions between M. aeruginosa and amoxicillin were significantly regulated by phosphorus (p < 0.05) and led to a complicated situation of combined pollution.