کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6972185 | 1453098 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A glutathione S-transferase from Proteus mirabilis involved in heavy metal resistance and its potential application in removal of Hg2+
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بهداشت و امنیت شیمی
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چکیده انگلیسی
Glutathione S-transferases (GSTs) are a family of multifunctional proteins playing important roles in detoxification of harmful physiological and xenobiotic compounds in organisms. In our study, a gene encoding a GST from Proteus mirabilis strain V7, gstPm-4, was cloned and conditionally expressed in Escherichia coli strain BL21(DE3). The purified GstPm-4 protein, with an estimated molecular mass of approximately 23Â kDa, was able to conjugate 1-chloro-2,4-dinitrobenzene and bind to the GSH-affinity matrix. Real-time reverse transcriptase PCR suggested that mRNA level of gstPm-4 was increased in the presence of CdCl2, CuCl2, HgCl2 and PbCl2, respectively. Correspondingly, overexpression of gstPm-4 in the genetically engineered bacterium Top10/pLacpGst exhibited higher heavy metal resistance compared to the control Top10/pLacP3. Another genetically engineered bacterium Top10/pBATGst, in which the DNA encoding GstPm-4 protein was fused with the DNA encoding Pfa1-based auto surface display system, was built. Top10/pBATGst could constitutively express the chimeric GstPm-4 and anchor it onto the cell surface subsequently. Almost 100% of the Hg2+ within the range of 0.1-100Â nM was adsorbed by Top10/pBATGst, and 80% of the bounded Hg2+ could be desorbed from bacterial cells when pH was adjusted to 6.0. Thus, Top10/pBATGst can be potentially used for efficient treatment of Hg2+-contaminated aquatic environment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hazardous Materials - Volume 261, 15 October 2013, Pages 646-652
Journal: Journal of Hazardous Materials - Volume 261, 15 October 2013, Pages 646-652
نویسندگان
Weiwei Zhang, Kun Yin, Bowei Li, Lingxin Chen,