Construction and evaluation of PAMAM-DOX conjugates with superior tumor recognition and intracellular acid-triggered drug release properties

- PAMAM-DOX conjugates (FPP-hyd-DOX) with different FA ratios were constructed and identified.
- In vitro evaluations exhibited the conjugates release DOX in an obvious pH-triggered manner.
- FPP-hyd-DOX was internalized by KB cells via clathrin-mediated endocytosis.
- Subcellular localization study revealed drug release of FPP-hyd-DOX was triggered in lysosomes.
- FPP-hyd-DOX conjugations would be a promising drug delivery carrier for targeted cancer therapy.