Crystallographic structure of Ni-Co coating on the affinity adsorption of histidine-tagged protein

- Ni-Co alloy coating for immobilizing histidine-tagged proteins.
- The stronger hcp texture results in better affinity adsorption to His-tagged biotin.
- Allotropic phase transformation to fcc leads to the decrease of affinity adsorption.
- Crystallographic structure of Ni-Co coating affects the specific affinity adsorption.