Polyvinylidene fluoride/silane-treated hydroxyapatite mixed matrix membrane for enzyme capturing

The silane coupling agent, N-(β-aminoethyl)-γ-aminopropyltrimethoxy silane (KH792), was employed to modify the surfaces of nano-hydroxyapatite (HAP) particles. Then the mixed matrix membranes (MMMs) were prepared by embedding HAP modified with KH792 (KH792-HAP) inside polyvinylidene fluoride (PVDF) matrix. The MMMs showed purification of enzyme via static adsorption and dynamic adsorption, and showed the potential of using MMMs for enzyme capturing in enzyme purification techniques. The MMMs concept combines adsorption and filtration in one step.