Formulation of glutathione responsive anti-proliferative nanoparticles from thiolated Akt1 siRNA and disulfide-crosslinked PEI for efficient anti-cancer gene therapy

- Thiol-modified siRNA (SH-siRNA) was delivered by bioreduciblepolyethylenimine (ssPEI).
- ssPEI/Akt1SH-siRNA complexes reduced proliferation and increased apoptosis of mouse colon cancer cells in vitro.
- The complexes reduced tumor proliferation and downregulation of Akt1 compared to controls in vivo.