Influence of micropattern width on differentiation of human mesenchymal stem cells to vascular smooth muscle cells

- A gradient micropattern of different stripe width was prepared.
- The width of micropattern stripes showed obvious effect on hMSCs orientation and differentiation.
- Narrow stripes allowed hMSCs to spread along the stripes and limited spreading in perpendicular direction.
- Cells cultured on the narrow stripes showed a significantly higher expression of VSMC marker protein.