Protein attachment to nanoporous anodic alumina for biotechnological applications: Influence of pore size, protein size and functionalization path

- The distribution of model proteins to nanoporous anodic alumina is studied.
- The protein attachment is analyzed in terms of functionalization path and pore size.
- Silane-PEG-NHS results in the most homogeneous distribution of collagen and BSA.
- APTMS-GTA tends to produce protein aggregates and a non-uniformly covered surface.
- These results can be useful in biosensing, 3D cell growth and controlled drug delivery.