Surface plasmon resonance biosensor for label-free and highly sensitive detection of point mutation using polymerization extension reaction

- A simple SPR biosensor strategy has been developed for rapid and highly sensitive detection of point mutation.
- This is the first application based on polymerization extension reaction for point mutation detection.
- 100 pM mutant sequences can be detected within 15 min.
- Wild-type and mutant-type sequences can be successfully discriminated.