Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine

- Nattokinase (NK) was efficiently adsorbed onto nanosilver (AgNPs).
- NK-AgNPs enhanced the heat stability and anticoagulant effect of NK.
- NK-AgNPs-PEI coating was prepared by layer-by-layer self-assembly.
- NK-AgNPs-PEI coating showed antithrombotic and antibacterial activity.