Improved double emulsion technology for fabricating autofluorescent microcapsules as novel ultrasonic/fluorescent dual-modality contrast agents

- An improved double emulsion technology with in situ reaction of lysine (Lys) and glutaraldehyde (GA) to fabricate autofluorescent PLGA microcapsules.
- A novel ultrasonic/fluorescent dual-modality contrast agents without conjugation to any fluorescent agent.
- This reaction product Lys-GA is found to exhibit bright and stable autofluorescence.
- The integration of the autofluorescence into ultrasonic contrast agent of PLGA microcapsules.