A novel method of AquaporinZ incorporation via binary-lipid Langmuir monolayers

► The incorporation of Histidine-tagged AquaporinZ in a lipid bilayer is investigated with Langmuir-Blodgett technology for the first time. ► Detergent removal in the Langmuir-Blodgett system is studied. ► AquaporinZ and detergent interaction during protein insertion is elaborated and a protein incorporation mechanism is proposed.