کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7001191 | 1454759 | 2018 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of silver nanoparticles with Lysozyme: Functional and structural investigations
ترجمه فارسی عنوان
تعامل نانوذرات نقره با لیزوزیم: تحقیقات کاربردی و ساختاری
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
چکیده انگلیسی
Biological implications of protein adsorption on nanoparticles' surfaces make interaction studies of paramount importance. Herein, mutual effects of lysozyme and silver nanoparticles on each counterpart have been studied using spectroscopic techniques. Surface Plasmon Resonance (SPR) studies showed decrease of interparticle distance upon interaction with biomolecule, and Dynamic Light Scattering (DLS) confirmed formation of nanoparticles aggregates. In consistency with SPR and DLS analyses, Scanning Electron Microscopy (SEM) showed that protein concentration plays a critical role either in guiding formation of stable complexes or dictating irreversible aggregations of nanoparticles. Analysis of Circular Dichroism (CD) spectroscopy data showed that protein retained most of its helical structure upon interaction with silver nanoparticles. Kinetic investigations also revealed that lysozyme almost maintained its catalytic activity, together with a slight decrease in the enzyme-substrate binding affinity. Results of this effort encourage combined utility of spectroscopic techniques to gain further insight into challenges at the interface for both bio and nano counterparts, prior to exploit variety of theranostic applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Surfaces and Interfaces - Volume 10, March 2018, Pages 216-221
Journal: Surfaces and Interfaces - Volume 10, March 2018, Pages 216-221
نویسندگان
Shahnaz Ashrafpour, Tahereh Tohidi Moghadam,